Contents

1. evaluation of homogenisation and lysis processes

• Evaluation key plant biomass/cell cultures

2. DNA extraction

• Chromosomal DNA from dried plant material
• Plasmid DNA from Escherichia coli after Birnboim-Doly
• Gel electrophoresis

3. restriction DNA

• Double digestion of plasmid DNA
• Gel electrophoresis
• Spectrophotometric determination of the DNA concentration

4. PCR polymerase chain reaction

• Amplification of different DNA samples
• Gel electrophoresis of the amplificates

5. transformation of Escherichia coli