Contents

Experiment 1: Theory and practice of the light microscope

• Microscopy of living microorganisms (native preparation) in bright field, with phase contrast and with oil immersion
• Production of a fixed preparation

Experiment 2: Sterile work in the microbiology laboratory

• Determination of the bacterial count in the air
• Sterile filtration
• Influence of different sterilisation times
• Influence of various disinfectants on the growth of microorganisms

Experiment 3: Growth and propagation

• Effect of the hydrogen ion concentration on the growth of microorganisms
• Effect of different NaCl concentrations and glucose concentrations on the growth of microorganisms
• Determination of the cell count with the THOMA counting chamber
• Colony count determination of various soil and liquid samples
• Degree of activation of a soil

Experiment 4: Isolation and identification

• Dilution smear
• Morphology
• Gram colouring
• KOH test
• Oxidase test

Experiment 5: Metabolic tests

• Starch hydrolysis
• Effect of microorganisms on the degradation of hydrocarbons
• Gas and acid formation

Experiment 6: Cultivation

• Bacterial growth curve (batch culture)